Sequence Analysis of the Arcelin-Phytohaemagglutinin-?-Amylase Inhibitor (APA) Locus in Three Phylogenetically Arrayed Phaseolus vulgaris Clones

APA is a multigene locus that comprises the protein genes of arcelin (Arc), phytohemagglutinin (PHA) or lectin, and an ?-amylase inhibitor (?-AI). These play essential roles in defense responses legume seeds to protect against seed weevils common bean (P. vulgaris). During evolution this complex locus, PHA proteins appeared first, followed by ?-AI Arc proteins. This study compared analyzed sequences three bacterial artificial chromosome (BAC) clones containing fragments genotypes G02771, BAT93 DGD1962. are recognized as representative crucial steps bean. Our findings demonstrated rearrangements during can be used characterize but concurrent adjacent gene regions on either side highly conserved. Part instability may due insertion retroelements conversion.